Key: Win : Draw : Loss
| Date | Home | Away | Ref | ||
|---|---|---|---|---|---|
| 08/08/2009 | Port Vale | 1 | 1 | Rochdale | J Waugh |
| 15/08/2009 | Bradford | 0 | 0 | Port Vale | D Whitestone |
| 18/08/2009 | Macclesfield | 2 | 0 | Port Vale | J Singh |
| 22/08/2009 | Port Vale | 1 | 0 | Darlington | P Tierney |
| 29/08/2009 | Hereford | 2 | 2 | Port Vale | G Scott |
| 05/09/2009 | Port Vale | 4 | 0 | Grimsby | G Eltringham |
| 12/09/2009 | Aldershot | 1 | 1 | Port Vale | P Crossley |
| 19/09/2009 | Port Vale | 0 | 1 | Bury | A D'Urso |
| 26/09/2009 | Notts County | 3 | 1 | Port Vale | A Taylor |
| 29/09/2009 | Port Vale | 2 | 2 | Accrington | D Sheldrake |
| 03/10/2009 | Port Vale | 0 | 0 | Bournemouth | R Shoebridge |
| 10/10/2009 | Shrewsbury | 0 | 1 | Port Vale | S Bratt |
| 17/10/2009 | Crewe | 1 | 2 | Port Vale | N Miller |
| 24/10/2009 | Port Vale | 1 | 1 | Cheltenham | S Cook |
| 31/10/2009 | Dag and Red | 1 | 1 | Port Vale | R East |
| 14/11/2009 | Port Vale | 1 | 2 | Rotherham | P Quinn |
| 21/11/2009 | Barnet | 0 | 0 | Port Vale | F Graham |
| 24/11/2009 | Port Vale | 2 | 2 | Torquay | J Moss |
| 01/12/2009 | Lincoln | 1 | 2 | Port Vale | R Booth |
| 05/12/2009 | Port Vale | 0 | 2 | Morecambe | K Stroud |
| 12/12/2009 | Northampton | 1 | 1 | Port Vale | M Russell |
| 28/12/2009 | Grimsby | 1 | 2 | Port Vale | G Sutton |
| 19/01/2010 | Port Vale | 3 | 1 | Burton | G Eltringham |
| 23/01/2010 | Port Vale | 0 | 0 | Macclesfield | P Miller |
| 26/01/2010 | Rochdale | 0 | 0 | Port Vale | C Pawson |
| 30/01/2010 | Port Vale | 2 | 0 | Hereford | G Horwood |
| 06/02/2010 | Burton | 1 | 0 | Port Vale | P Crossley |
| 09/02/2010 | Port Vale | 1 | 2 | Chesterfield | P Taylor |
| 13/02/2010 | Torquay | 1 | 2 | Port Vale | G Scott |
| 20/02/2010 | Port Vale | 0 | 2 | Barnet | C Dunn |
| 23/02/2010 | Port Vale | 4 | 0 | Lincoln | P Gibbs |
| 27/02/2010 | Morecambe | 1 | 0 | Port Vale | J Waugh |
| 02/03/2010 | Darlington | 1 | 3 | Port Vale | M Haywood |
| 06/03/2010 | Port Vale | 1 | 3 | Northampton | D Webb |
| 09/03/2010 | Port Vale | 2 | 1 | Bradford | N Swarbrick |
| 13/03/2010 | Chesterfield | 0 | 5 | Port Vale | P Tierney |
| 20/03/2010 | Cheltenham | 1 | 1 | Port Vale | G Ward |
| 27/03/2010 | Port Vale | 0 | 1 | Crewe | C Sarginson |
| 03/04/2010 | Rotherham | 1 | 2 | Port Vale | M Halsey |
| 05/04/2010 | Port Vale | 3 | 1 | Dag and Red | G Sutton |
| 10/04/2010 | Port Vale | 1 | 1 | Aldershot | D Whitestone |
| 13/04/2010 | Accrington | 1 | 2 | Port Vale | J Moss |
| 17/04/2010 | Bury | 1 | 1 | Port Vale | D Foster |
| 24/04/2010 | Port Vale | 2 | 1 | Notts County | K Evans |
| 01/05/2010 | Bournemouth | 4 | 0 | Port Vale | S Hooper |
| 08/05/2010 | Port Vale | 1 | 1 | Shrewsbury | K Hill |